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ldh level in ac16 cells measured using promega cytotox 96® kit  (Promega)
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Promega ldh level in ac16 cells measured using promega cytotox 96® kit
Deficiency of RBM15 ameliorated H/R-stimulated oxidative damage <t>in</t> <t>AC16</t> cells. AC16 cells were treated with H/R, H/R + si-NC or H/R + si-RBM15 and untreated cells were control. ( A and B ) The mRNA and protein levels in AC16 cells were measured by qRT-PCR assay and western blot assay, respectively. ( C ) AC16 cell viability was explored by CCK-8 assay. ( D - G ) The levels of <t>LDH,</t> ROS, MDA and SOD in AC16 cells were examined by related commercial kits. ** P < 0.01, *** P < 0.001
Ldh Level In Ac16 Cells Measured Using Promega Cytotox 96® Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Deficiency of RBM15 ameliorated H/R-stimulated oxidative damage in AC16 cells. AC16 cells were treated with H/R, H/R + si-NC or H/R + si-RBM15 and untreated cells were control. ( A and B ) The mRNA and protein levels in AC16 cells were measured by qRT-PCR assay and western blot assay, respectively. ( C ) AC16 cell viability was explored by CCK-8 assay. ( D - G ) The levels of LDH, ROS, MDA and SOD in AC16 cells were examined by related commercial kits. ** P < 0.01, *** P < 0.001

Journal: Hereditas

Article Title: RBM15 promotes hypoxia/reoxygenation-induced ferroptosis in human cardiomyocytes by mediating m6A modification of ACSL4

doi: 10.1186/s41065-025-00453-0

Figure Lengend Snippet: Deficiency of RBM15 ameliorated H/R-stimulated oxidative damage in AC16 cells. AC16 cells were treated with H/R, H/R + si-NC or H/R + si-RBM15 and untreated cells were control. ( A and B ) The mRNA and protein levels in AC16 cells were measured by qRT-PCR assay and western blot assay, respectively. ( C ) AC16 cell viability was explored by CCK-8 assay. ( D - G ) The levels of LDH, ROS, MDA and SOD in AC16 cells were examined by related commercial kits. ** P < 0.01, *** P < 0.001

Article Snippet: LDH level in AC16 cells was measured using Promega CytoTox 96 ® kit (Promega, Madison, WI, USA) in line with the protocols.

Techniques: Control, Quantitative RT-PCR, Western Blot, CCK-8 Assay

RBM15 knockdown repressed H/R-induced AC16 cell damage by altering ACSL4 expression. ( A ) The protein level of ACSL4 in AC16 cells transfected with pcDNA-NC or pcDNA-ACSL4 was measured by western blot. ( B - K ) AC16 cells were divided into 5 groups: control, H/R + si-NC, H/R + si-RBM15, H/R + si-RBM15 + pcDNA-NC and H/R + si-RBM15 + pcDNA-ACSL4. ( B ) AC16 cell viability was assessed by CCK-8 assay. ( C - G ) The levels of LDH, ROS, MDA, SOD and Fe 2+ in AC16 cells were examined by commercial kits. ( H ) The protein levels of GPX4, ACSL4, FTH1 and NCOA4 in AC16 cells were measured by western blot. ( I - K ) The levels of GSH, GSSG and GSH/GSSG in AC16 cells were examined by the indicated kits. * P < 0.05, ** P < 0.01, *** P < 0.001

Journal: Hereditas

Article Title: RBM15 promotes hypoxia/reoxygenation-induced ferroptosis in human cardiomyocytes by mediating m6A modification of ACSL4

doi: 10.1186/s41065-025-00453-0

Figure Lengend Snippet: RBM15 knockdown repressed H/R-induced AC16 cell damage by altering ACSL4 expression. ( A ) The protein level of ACSL4 in AC16 cells transfected with pcDNA-NC or pcDNA-ACSL4 was measured by western blot. ( B - K ) AC16 cells were divided into 5 groups: control, H/R + si-NC, H/R + si-RBM15, H/R + si-RBM15 + pcDNA-NC and H/R + si-RBM15 + pcDNA-ACSL4. ( B ) AC16 cell viability was assessed by CCK-8 assay. ( C - G ) The levels of LDH, ROS, MDA, SOD and Fe 2+ in AC16 cells were examined by commercial kits. ( H ) The protein levels of GPX4, ACSL4, FTH1 and NCOA4 in AC16 cells were measured by western blot. ( I - K ) The levels of GSH, GSSG and GSH/GSSG in AC16 cells were examined by the indicated kits. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: LDH level in AC16 cells was measured using Promega CytoTox 96 ® kit (Promega, Madison, WI, USA) in line with the protocols.

Techniques: Knockdown, Expressing, Transfection, Western Blot, Control, CCK-8 Assay